重组白细胞介素6促进骨髓移植小鼠的免疫功能重建

In vivo administration of recombinant IL—6 accelerates the immunological reconstitution of mice following bone marrow transplantation

本文研究了重组白细胞介素6(rIL—6)对骨髓移植小鼠免疫功能重建的作用。结果表明,经致死量(950Rab)照射的 BA(?)b/c 小鼠在骨髓移植后第二天开始腹腔注射100μg/kg/天剂量的 rL—6,在骨髓移植后30天,其脾细胞对 conA 和 Lps 的增殖反应,对异型脾细胞(C_(57)BL/6)的混合淋巴细胞反应(MLR)和细胞杀伤活性(CTL)以及对绵羊红细胞(SRBC)的溶血空斑数(PFC)均较正常骨髓移植小鼠明显增强。骨髓移植后用 rIL—610天的小鼠,在移植后30天时,上述各项免疫功能已达到正常骨髓移植小鼠60天时的水平,说明 rIL—6能明显加速骨髓移植后免疫功能的重建过程.

In this study the effect of rIL—6 on the immunological reconstitution of mice following bone marrow transplantation(BMT)was evaluated.The lethally irradiated(950Rad)BALB/c mice engrafted with BMT were injected ip with rIL—6(1000 ug/kg)on day 2.At day 30 after BMT the proliferative responses to ConA and LPS,the mixde lymphocyte reaction(MLR)and the activity of cytotoxic T lymphocytes(CTL)to the allogeneic splenic cells(C57BL/6),and the numbers of plaque forming cells(PFC)to SRBC were significantly enhanced in the rIL—6 treated recipient mice,as compared to that in the control BMT mice.In the BMT mice following 10 day rIL—6 treatment,the above immune functions on day 30 after BMT were recovered to the immunological condition of the control BMT mice on day 60,suggesting that the rIL—6 is able to accelerate the process of immunological reconstitution of BMT mice.