白细胞介素1刺激系膜细胞IL—1转化生长因子β基因表达

The possible role of IL—1 autocrine and paracrine in mesangial cell(MSc)

本文应用分子生物学技术证明培养的肾小球系膜细胞有 TGFβmRNA 基因表达。并且 IL—1可促进系膜细胞 IL—1、TGFβ基因表达,提示细胞因子的自分泌和旁分泌在肾小球炎症硬化过程中起着重要作用.

It was reported that autocrine and paracrine of cytokines play a central role in the pathogenesis of glomeru- lonephritis(GN)and glomerulosclerosis(GS).previous studies demanstrated that IL—1 like factor is not only produced by MSc,but also stimulates MSc proliferation.In order to examine the autocrine and paracrine of IL —1 in MSc in molecular biologic level,we explored the effects of rIL—1 on MSc IL—1 and TGFB mRNA gene expression.Total RNA was extracted from subcultured MSc in presence of absence of rIL-1 in 3,6,12,hours and dot blots.The MSc proiferation was a dose dependent of rIL—1,and IL—1 promoted MSc IL—1 and TGFB mRNA gene expression.Our results support the possible role of IL—1 autocrine and paracine in MSc.