人原癌基因c—fos反义RNA制备及初步应用

Preparation and primary application of human c—fos antisense RNA

采用逆转录 DNA 聚合酶链反应方法合成了人原癌基因 c—fos 特定 cDNA 片段.然后,将该片段与 PGEM3Zf(+)质粒重组,构建成为可体外转录 c—fos 反义 RNA 重组质粒.选用 T7RNA 聚合酶系统合成了 c—fos 反义 RNA。且本文对反义 RNA 的应用亦进行了初步探讨.

The cDNA fragment of human c—fos oncogene has been obtained by a reverse transcription—DNA poly- merase chain reaction.Then the cDNA was ligated into pGEM3Zf(+)plasmid cut with Sma Ⅰ.with the re- combined plasmid,c—fos antisense RNA was synthesized in T7 RNA polymerase system.The primary appli- cation of anti-sense RNA is also studied in this article.