超重症联合免疫缺陷小鼠体液免疫功能特征研究

Humoral Immunologic characteristics of super severe combined Immunodeficient mice

本文对超重症联合免疫缺陷小鼠(B.C.B—17scid—beige)的体液免疫功能特征进行了研究,结果如下:1.血清中免疫球蛋白含量低于0.01mg/ml,与正常对照小鼠差异非常显著(p<0.01),2.脾脏发育异常,器官萎缩,B 细胞区细胞稀疏,3.脾脏 B 淋巴细胞缺乏:B220阳性细胞为0.2±0.1%、SmIg 阳性细胞为0.7±0.2%,4.脾淋巴细胞不能针对 B 细胞有丝分裂原细菌脂多糖(LPS)的刺激发生增殖,~3H—Tdr 参入值仅为549.33±146.22cpm,明显低于正常对照小鼠(p<0.01)。上述结果表明该鼠继承了其亲代重症联合免疫缺陷小鼠(scid)的体液免疫缺陷特征,从而为人源单抗及其它基因工程产品的生产提供了更为理想的工具.

The super severe combined lmmunodeficient mice(B.C.B—17scid—beige mice)showed severe humoral im- munodeficiency.The mouse strain has a low concentration of Ig(below 0.01mg/ml,vs 5 mg/ml for control nor- mal mice(p<0.01);low number of B cell(devoid of lymphocytes in spleenic corpuscle,B_(220)+~cell 0.24±0.1%, vs19.1±1.8%for control BALB/c mice and SmIg^+ cell 0.7±0.1%,vs 60.5±3.1% for BALB/c mice(p<0. 01)The spleen cells of B.C.B—17acid—beige mice lacked responsiveness to B cell mitogen(lipopolysaccharide 10μg/ml,549.33±146.22 cpm)with the level comparable to C.B—17acid mice(198.67±34.02 cpm,at 10μg/ml LPS,p<0.05),but much lower than control mice(7798.39±1782.91 at 10μg/ml LPS,p<0.01), All these results indicated that the mice inherited humoral immunologic characteristics of acid gene.The new mice strain should be a more perfect animal host for production of human monoclonal antibody and genetic engi- neering products.