离子色谱法测定饲料中氯化胆碱和三甲胺的含量

Determination of Choline Chloride and Trimethylamine in Feedstuff by Ion Chromatography

建立了离子色谱法测定饲料中氯化胆碱含量及鉴别饲料中氯化胆碱及掺假物三甲胺的方法。选用IonPacCS12阳离子交换色谱柱(250mm×4mmi d )和8 5mmol/LH2SO4淋洗液,抑制型电导检测,在16min内分离测定了包括胆碱和三甲胺在内的8种阳离子。胆碱和三甲胺的最小检出限分别为0 1mg/L和0 05mg/L。方法回收率为99 25%～102 5%。该方法具有灵敏度高、选择性强、操作简单等优点。

A method was developed for the determination of choline chloride (CC) and trimethylamine (TMA) in feedstuff by ion chromatography. The separation of eight ions including Li^+, Na^+, NH^+_4, K^+, TMA, choline, Mg^(2+) and Ca^(2+) was achieved by using an IonPac CS12 column (250 mm×4 mm i.d.) and 8.5 (mmol/L) H_2SO_4 as eluent. Cations were detected by suppressed conductivity detection. The limits of detection of CC and TMA were 0.1 (mg/L) and 0.05 (mg/L), respectively. The method recoveries were between 99.25% and 102.5%. The method was sensitive, selective, and simple. The results of sample analysis showed that the method was very useful for the authenticating of choline chloride in feedstuffs.