脑热清对EP性发热家兔下丘脑、脑脊液cAMP及腹中隔区AVP含量的影响

Effects of Naoreqing Oral Solution on cAMP in the Hypothalamus and Cerebrospinal Fluid and AVP in the Ventral Septal Area in the Rabbits with Fever Induced by EP

目的 探讨脑热清口服液的解热机制。方法 建立内生致热原 (EP)性发热家兔模型 ,观察脑热清对家兔体温的影响 ,并用放射免疫法检测下丘脑和脑脊液中cAMP及腹中隔区精氨酸加压素 (AVP)含量的变化。结果 EP组的ΔT及TRI4 变化、下丘脑及脑脊液中cAMP含量、腹中隔区AVP含量显著高于脑热清 +EP组 (P <0 0 1 )。 4组的体温变化分别与下丘脑和脑脊液中cAMP以及腹中隔区AVP的变化呈正相关 (P <0 0 1 )。结论 脑热清的解热机制可能是通过抑制下丘脑cAMP的生成与释放 。

Objective To investigate the antifebrile mechanism of Naoreqing Oral Solution (NOS). Method Established by the injection of endogenous pyrogen (EP) was the rabbit model of fever, with which the effect of NOS on the rabbit body temperature was observed; and the effects of NOS on the levels of cAMP in the hypothalamus and cerebrospinal fluid and arginine vasopressin (AVP) in the ventral septal area were detected by the radioimmunoassay. Results The body temperature and the levels of TRI 4, cAMP in the hypothalamus and cerebrospinal fluid and AVP in the ventral septal area in the rabbits in the EP group were significantly higher than those in the rabbits in the EP plus NOS group ( P <0 01). The change in the body temperature was in a positive correlation with the change in the levels of cAMP in the hypothalamus and cerebrospinal fluid and AVP in the ventral septal area in all the four animal groups in the experiment ( P <0 01). Conclusion The antifebrile mechanism of NOS may be both inhibiting the production and release of cAMP in the hypothalamus and promoting the release of AVP in the ventral septal area.