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威麦宁对小鼠Lewis肺癌细胞周期的影响 被引量:12

Influence of Weimaining on the Cell Cycle of Lewis Lung Carcinoma in Mice
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摘要 目的 复制小鼠Lewis肺癌模型,检测威麦宁的抑瘤率。方法 采用流式细胞术分析、免疫组化法检测药物对模型鼠肿瘤细胞周期及对CyclinD1表达的影响。结果 威麦宁浓度在 1 0 0、1 5 0、2 0 0、2 5 0mg/(kg·d)时,对小鼠Lewis肺癌的抑制率分别为 1 9 1 4 %、33 5 9%、4 0 6 3%、5 1 5 6 %,药物对肿瘤的抑制作用具有剂量依赖性;药物可将肿瘤细胞阻滞在G0 ~G1期,并降低肿瘤细胞CyclinD1的表达(P <0 0 1)。结论 威麦宁可能是通过降低肿瘤细胞CyclinD1的表达,将肿瘤细胞阻止于G0 ~G1期,使细胞不能进入S期进行DNA复制,从而抑制肿瘤的生长。 Objective To establish the mouse model of Lewis lung carcinoma and detect the carcinoma inhibiting rate of Weimaining. Methods The flow cytometry and immunohistochemical staining were used to investigate the effects of Weimaining on the cell cycle of carcinoma and the expression of cyclin D1 in the model mice. Results It was showed that the carcinoma inhibiting rates of Weimaining were 19 14%, 33 59%, 40 63% and 51 56%, respectively when it was given at the doses of 100 mg/kg/d, 150 mg/kg/d, 200 mg/kg/d and 250 mg/kg/d, respectively to the model mice, and the effects were exerted in a dose dependent manner. Weimaining could halt the growth of the carcinoma cells in the G 0-G 1 phase and decrease the expression of cyclin D 1 ( P <0 01). Conclusion One of the Lewis lung carcinoma inhibiting mechanisms of Weimaining may be decreasing the expression of cyclin D 1 in the carcinoma cells and halting the growth of the carcinoma cells in the G 0-G 1 phase, and hence, making them unable to develop into the S phase for DNA replication.
出处 《北京中医药大学学报》 CAS CSCD 北大核心 2004年第2期41-44,共4页 Journal of Beijing University of Traditional Chinese Medicine
基金 国家中医药管理局课题(No.2 0 0 0 J B 0 2 )
关键词 威麦宁 LEWIS肺癌 细胞周期蛋白D1 小鼠 Weimaining Lewis Lung Carcinoma Cyclin D 1 Mice
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