家蚕母性基因的表达序列标签分析

Analysis of silkworm maternal genes with expressed sequence tag

为了研究家蚕Bombyxmori卵中储存的母性遗传信息 ,以未受精的肾脏型和正常型蚕卵为材料构建了cDNA文库 ,并对其随机测序 ,共获得 391条表达序列标签 (EST)序列 ,经拼接后得到 374条非重复序列。生物信息学分析发现 ,172个非重复序列与国际数据库GenBank和silkbase中的已知基因具有较高的相似性。除 3个已知母性基因外 ,其余主要与DNA复制、能量代谢、信号转导、转录、蛋白质结构以及胚胎发育中个体形成等相关。

The cDNA libraries were constructed from the kidney and normal unfertilized eggs of the silkworm, Bombyx mori and sequenced at random in order to study the maternal genetic information contained in the silkworms eggs. We had successfully sequenced 391 ESTs (expressed sequence tag) and obtained 374 unigenes after assembling. Bioinformatics analysis indicated 172 unigenes had significant similarity with the known genes recorded in GenBank and silkbase. Except three known maternal genes, the rest were mainly related to replication, metabolism,signal transduction, transcription factor, protein structure and embryo development. These results will provide source for further study on the development regulation of early embryo of the silkworm.