光肩星天牛种群间及其近缘种遗传关系的RAPD研究(英文)

Characterizing populations of Anoplophora glabripennis and related taxa with RAPD

利用RAPD技术对采自中国和美国的星天牛属Anoplophora 5个种及 8个光肩星天牛Anoplophoraglabripennis(Motschulsky)地理种群共 13个样品进行了遗传相似性分析。选用了Operon公司生产的引物H系列 2 0个 ,L系列 2 0个 ,Q系列11个共 5 1个引物 ,最后从 4 0个引物中筛选出 2 6个具有多态性的引物作为第一组用于星天牛属种间和光肩星天牛种群间分析 ,从 31个引物中筛选出 19个具有多态性的引物作为第二组单独用于光肩星天牛种群分析。根据第一组引物实验获得的RAPD聚类图及遗传距离表明 ,各个地理种群的光肩星天牛和黄斑星天牛A .nobilis都聚在一起 ,形成一个大的分枝 ,而四川星天牛A .freyi、楝星天牛A .horsfieldi和星天牛A .chinensis均在此分枝之外。来自美国纽约和芝加哥的光肩星天牛种群聚于中国光肩星天牛种群之外的另一个独立的分枝上。分布在我国宁夏、内蒙古和河北的光肩星天牛以及宁夏黄斑星天牛和山东、陕西的光肩星天牛分别聚在一起 ,而甘肃的光肩星天牛与甘肃的黄斑星天牛则聚于另一枝 ,且它们之间的遗传距离很近 ,仅为 0 132 4 ,说明这两者之间有着极其相近的亲缘关系 ,由此推断光肩星天牛和黄斑星天牛的差异很小 ,遗传关系难以区分 ,进一步证实了它们很可能是同一个种下的两个不同的型。

Five Anoplophora sibling species and eight geographical populations of the Asian longhorned beetle (ALB), Anoplophora glabripennis (Motsch.) collected from China and the USA were analyzed using the random amplified polymorphic DNA (RAPD) method. A total of 51 random primers (20 OPH, 20 OPL, 11 OPQ kits) were selected,of the 40 primers used in primer group Ⅰ, 26 primers produced polymorphic bands in phylogeny analyses of Anoplophora species and A.glabripennis populations. In primer group Ⅱ, 19 of the 31 primers produced polymorphic bands in A. glabripennis population analyses. Based on the computer-generated RAPD cladogram using primer group Ⅰ, eight geographical populations of A. glabripennis and two populations of A. nobilis can be grouped in one phylogenic cluster that is different from the other Anoplophora species. Six geographical populations of A. glabripennis in China form a cluster branch, which can be divided into two sub-branches: one sub-branch consists of populations from the provinces of Shaanxi, Shandong, Hebei, Nei Mongol, and Ningxia, and the other consists of A. glabripennis population from Gansu Province. The two A. glabripennis populations from New York and Chicago can be considered as an independent branch of a cluster with 0.2525 genetic distances between them. Similar results were also obtained with A. glabripennis geographical populations using primer group Ⅱ. These results indicated that the specimens of A. glabripennis populations collected from the USA were somewhat different genetically from specimens collected from some parts of China. The cladogram showed that A. nobilis populations from Gansu and Ningxia were mixed with the A. glabripennis cluster with little difference between the two species, which strongly supports the findings that the two may in fact be a single species.