葛根素对慢性低氧性肺动脉高压大鼠肺血管重构的影响

Effects of puerarin on pulmonary vascular structure of chronic hypoxia in rats

目的 观察葛根素对慢性低氧性肺动脉高压大鼠肺动脉压力和肺血管重构的调节作用。方法 SD大鼠 3 0只随机分为 :正常对照组 (NC)、低氧组 (HH)、低氧加葛根素组(HP)。分别测定平均肺动脉压 (mPAP) ;肺组织匀浆超氧化物歧化酶 (SOD)活性、丙二醛 (MDA )含量 ;血浆内皮素 1(ET 1)、一氧化氮代谢产物 (NO-x )含量 ;弹力纤维染色法测定肌型动脉百分比 ,肺中、小动脉相对中膜面积 (RMP)、厚度 (RMT) ;采用免疫组织化学法检测Ⅰ、Ⅲ型胶原蛋白的表达 ;核酸原位杂交法检测肺动脉Ⅰ型前胶原mRNA表达。结果 HP组与HH组相比 :①mPAP降低 (P <0 0 1) ;平均颈动脉压 (mCAP)差异无显著性 (P >0 0 5) ;②肺组织匀浆SOD活性升高 (P <0 0 1) ,MDA含量降低 (P <0 0 1) ;③血浆ET 1浓度降低 (P <0 0 1) ,NO-x 含量升高 (P <0 0 1) ,ET 1/NO-x 比值降低 (P <0 0 1) ;④肌型动脉百分比和肺中、小动脉RMP及RMT降低 (P <0 0 1) ;⑤肺细小动脉Ⅰ型胶原蛋白及Ⅰ型前胶原mRNA表达降低 (P <0 0 1) ,而Ⅲ胶原蛋白平均吸光度值差异无显著性 (P >0 0 5)。结论 葛根素注射液可预防慢性低氧性肺动脉高压大鼠的形成 ,减轻肺血管重构。其机制可能与抗氧化作用 ,调节肺动脉胶原蛋白合成以及恢复ET

AIMTo investigate the effects of puerarin on pulmonary vascular structure in pulmonary hypertension rats induced by chronic hypoxia. METHODSThirty male spragu-dawley rats were randomly divided int o three groups:control group(NC), hypoxia group(HH), hypoxia+puerarin group(HP). Collagen Ⅰ, Ⅲ and collagen I mRNA were observed in pulmonary arterioles by th e technique of immunohistochemistry and in situ hybridization. RESULTS①mPAP was much higher in rats of HH group than that of NC group(P<0 01), and lower in HP group than that in HH group(P<0 01). Differences of mC AP were not found among three groups(P>0 05). ②The contents of lung homoge nates SOD and MDA of HP group were obviously higher than those of HH group(P <0 01). ③The levels of plasma ET-1 and NO were much higher in rats of HH group than those of NC group(P<0 01),[FQ(10。46,X-WZ]and lower in HP group than those in HH group (P<0 01). ④Percentage of muscularized artery, relative media t hickness(RMT) and relative media area(RMA) were much higher in HH group than tho se of NC group, HP group were much slighter than those of HH group(P<0 01). ⑤Expression of collagen I and collagen I mRNA in pulmonary arterioles were sig nificantly higher in rats of HH groups than those of NC group (P<0 01),and they were lower in rats of HP group than those of HH group (P<0 01). Expre ssion of collagen Ⅲ was no difference among three groups(P>0 05). CONCLUSIONPuerarin can improve pulmonary vessel remodeling.