小蓬草总黄酮提取条件及含量测定

Study on Extraction and Determining the Content of Total Flavones in Cronyza canadensis

采用聚酰胺吸附-硝酸铝显色法,以芦丁为标样,在510nm处测总黄酮含量,通过正交实验,在不同乙醇体积分数(50%、65%、80%),不同固液比(1∶20、1∶35、1∶50),不同提取时间(1h、2h、3h)和不同提取温度(40℃、60℃、80℃)条件下,得出小蓬草叶总黄酮提取最佳条件为:在80℃条件下乙醇体积分数65%,固液比1∶50,提取3h.并用该最佳提取条件分别提取小蓬草根、茎、叶中的黄酮类化合物,测其含量分别为0.773%、0.380%、1.185%.

Extract total flavones from leaves of Cronyza canadensis and purified by polycaprolactam, produced color with aluminium nitrate that was quantitated by measurement of the absorbance at 510 nm. The rutin was used as a standard compound .Discussing the primary factors which influences extraction ratio, those are,ethanol ancentration(50%,65%,80%),solid-liquid ratio(1∶20,(1∶35),1∶50),extraction time (1 h,2 h,3 h), and extraction temperature(40℃,60℃,80℃) and analyzing preferable extraction conditions by variance analysis and orthogonal design, the results show that the optimal conditions for extraction from leaves are 65% ethanol, solid-liquid ratio 1∶50 and extract for 3 h at 80℃. Extraction total flavones from leaves,stems and roots by the optimal conditions and determine content.