摘要
用SDS高盐提取介质简便快速提取建兰(Cymbidiumensifolium)嫩叶DNA.提得的DNA分子大小约为48kb,A260/A280=1.77～1.86,DNA得率为每g鲜叶410～1145μg.提取的DNA无需经RNase处理,可直接用于限制性内切酶酶切和随机扩增多态DNA反应.
A fast and convenient method for extraction of DNA in young fresh leaves of Cymbidium ensifolium was introduced.The isolated DNA was 48 kb, and the A_(260)/A_(280) of DNA was 1.77～1.86. The production rate of DNA was 410～1145 μg/g. The DNA was suitable for digesting with restriction enzyme and Random Amplified Polymorphic DNA reaction without purified with RNase.
出处
《福建师范大学学报（自然科学版）》
CAS
CSCD
2004年第1期118-120,共3页
Journal of Fujian Normal University：Natural Science Edition
基金
福建省教育厅基金资助项目(JA02187)
关键词
建兰
DNA
提取方法
RAPD
分子生物学
Cymbidium ensifolium
DNA extraction
Random Amplified Polymorphic DNA (RAPD)
