混合植物甾醇乙酰化物的反相高效液相色谱分析

HPLC Analysis of Phytosterol Acetates

应用高效液相色谱对豆甾醇乙酸酯、β_谷甾醇乙酸酯和混合甾醇乙酸酯进行了分析测定 ;实验采用的色谱柱为HypersilODS反相柱(4.6mm×150mm ,5μm) ,流动相为色谱纯甲醇 ,紫外检测波长为210nm ,恒溶剂洗脱 ;实验结果表明 ,混合植物甾醇及其乙酸酯的响应时间在25min以内 ,且各种甾醇及其酯的分离效果较好 ,采用峰面积归一法能够快速准确地测定混合甾醇中豆甾醇乙酸酯和 β_谷甾醇乙酸酯的含量。

Stigmasterol acetate, β-sitosterol acetate and mixed phytosterol acetates were analyzed by HPLC. Chromatographic separation was carried out with a Hypersil ODS column(4.6 mm×150 mm, 5 μm) under isocratic conditions with methanol as the mobile phase at a flow-rate of 0.7 mL/min. The UV detection wavelength was set at 210 nm. The experimental results showed that phytosterol acetates could be separated within 25 minutes and determined accurately by the peak-area normalization method.