bFGF在高血压大鼠不同时期心肌细胞表达变化的研究

STUDY ON THE EXPRESSIVE CHANGES OF BFGF IN HYPERTENSIVE RAT’S CARDIAC MUSCLE CELLS DURING DIFFERENT PHASES

目的 :以Dahl高血压大鼠为研究对象 ,观察碱性成纤维生长因子 (bFGF)在高血压不同阶段心肌细胞的分布变化 ,以期为高血压性心脏病的研究提供理论基础和新治疗途径。方法 :通过免疫组织化学方法 ,观察bFGF在心肌组织切片中的表达变化情况 ,计算单位面积心肌组织bFGF的阳性细胞数。结果 :bFGF免疫细胞在DahlS和DahlR组大鼠的心肌细胞中均有表达 ,DahlS组bFGF阳性细胞数均较同时期DahlR组增多 (P <0 .0 1 ) ,DahlS组自 1 0周起至 1 2周bFGF阳性细胞数逐渐增多 (P <0 .0 1 ) ,DahlR组在高血压不同时期bFGF无显著性变化 (P >0 .0 5 )。结论 :在高血压和血压正常时 ,bFGF在心肌细胞上均有表达 ,并随高血压的病程延长呈上升趋势。

Objective:The basic fibroblast growth factor (bFGF) can exert remarkable influence on the migration,differentiation and proliferation of cells.This study is aimed to explore the distribution bFGF in rat's cardiac muscle cells in different phases of hypertension by way of immunohistochemistry.Methods:The expressive changes of bFGF in tissue section were investigated by means of immunohistochemistry.The number of bFGF positive cells within unit area was counted.Results:The bFGF was expressed in the cardiac muscle cell of the two groups.bFGF positive cells in Dahl-S groups were much higher than that in the Dahl-R group in the same period(P<0.01).The number of bFGF positive cells also increased gradually (P<0.01).There were no obvious changes about bFGF in different period of hypertension in DahlR group(P>0.05).Conclusions:The expression of the bFGF occurred in the cardiac muscle cells at hypertension,the trend of expression intensified with prolonging of hypertension process.The results indicated that bFGF participated and played important role in hypertrophic reaction.