2型糖尿病伴周围神经病变患者外周血单个核细胞中醛糖还原酶mRNA水平测定

Aldose reductase gene expression in peripheral blood mononuclear cells and its association with peripheral neuropathy in type 2 diabetic patients

目的探讨外周血单个核细胞(PBMC)中醛糖还原酶(AR)mRNA水平与2型糖尿病并发周围神经病变的关系。方法用RT-PCR法检测32例2型糖尿病患者及35名正常对照者PBMC中ARmRNA水平,用肌电图检测腓神经传导速度,根据神经传导速度有无减慢将糖尿病患者分为普通糖尿病组和糖尿病神经病变组。结果2型糖尿病患者PBMC中ARmRNA的表达量(0.59±0.56)高于正常对照组(0.30±0.11),P=0.003;糖尿病是否伴神经病变患者之间ARmRNA的表达量无差别。结论2型糖尿病患者PBMC中ARmRNA表达水平增高与是否伴神经病变无关。

Objective To investigate the relationship between the levels of aldose reductase(AR) mRNA in peripheral blood mononuclear cells (PBMC) and the peripheral neuropathy in type 2 diabetic patients. Methods The levels of AR mRNA in PBMC was determined by RT-PCR, and the nerve conduction velocity (NCV) in the calf nerve was measured. A total of 32 patients with type 2 diabetes and 35 normal controls were studied. The diabetic patients were divided into 2 groups patients with normal NCV and those with delayed NCV. Results AR mRNA levels in PBMC of type 2 diabetic patients was significantly higher than those of normal controls (0.59±0.56 vs 0.30±0.11, P=0.003). There was no significant difference in AR mRNA levels between two groups of diabetics.Conclusion Type 2 diabetic patients have a significantly higher expression of AR mRNA in PBMC than that in normal controls, but that is not related to the presence of diabetic peripheral neuropathy.