酒精性肝病大鼠肝脏组织中铁的测定

Iron involved in the mechanisms of alcoholic liver disease

目的研究铁与酒精性肝病的关系,以进一步探索铁在该病发病机制中的作用。方法采用酒精灌胃法建立酒精性肝病大鼠模型,取血标本检测肝功能,取肝脏标本作HE染色和普鲁氏蓝染色。结果各酒精灌胃大鼠肝组织均有不同程度的脂肪变性和炎症,与对照组相比,差别有显著性意义(均P<0.05)。实验组大鼠肝脏铁染色阳性铁颗粒较对照组明显增多,并与酒精灌胃时间和肝功能损伤程度呈正相关(均P<0.05)。结论铁在酒精性肝病大鼠肝脏沉积明显增多,可能参与酒精性肝病的发生和发展。

Objective To evaluate the relationship between the hepatic iron deposit and the degree of alcoholic liver injury. Methods Sprague-Dawley rats were fed 56%(v/v) ethanol by gastric lavage, at a dose of 8g/kg.day for 4, and 12 weeks. At 0, 4, and 12 weeks, blood samples were collected and then the rats were sacrificed. The liver specimens were examined with HE staining for hepatic steatosis and alcoholic hepatitis, and the level of hepatic iron content was examined using Perls' Prussian-blue method. Results Histological changes of chronic liver injury indicated that the degree of alcoholic liver injury increased with the duration of alcohol consumption. The score of hepatic iron content in alcohol group was higher than the control group, and the difference was significant. Conclusion Chronic alcohol consumption may lead to liver injury. The hepatic iron content appears to be correlated with the degree of alcoholic liver injury, it indicates that iron deposit may be involved in the pathogenesis of alcoholic liver disease.