摘要
目的 研究梯度降温、深低温保存同种骨组织的生物力学特性 ,为临床应用理想的同种骨移植材料修复各种原因引起的骨缺损提供理论依据。方法 将新鲜尸体的股骨经过预处理后 ,梯度降温 (- 2℃ /min)至 - 6 0℃ ,投入 - 80℃深低温冰箱中保存。一月后取出 ,进行生物力学实验。结果 实验得出的数据与以往研究新鲜骨的数据十分接近。结论 梯度降温、深低温保存的同种骨保持与新鲜骨最为接近的生物力学特性 。
Objective To study the biomechanics of allograft bone tissue, and to provide a suitable preservation method for bone allograft. Methods The femora of fresh corpse were gradient frozen (-2℃/min) to -60℃ after the pretreatment. And then put them into the cryopreservation refrigerator of -80℃. One month later, the biomechanical tests were carried on. Results The results of the biomechanical test were close to the data of fresh bone in former research. Conclusion The biomechanical characteristics of gradient freezing and cryopreservation bone allograft were close to the fresh bone. The method of this preservation could provide gist to the intending further research.
出处
《医用生物力学》
EI
CAS
CSCD
2004年第1期48-50,共3页
Journal of Medical Biomechanics
关键词
制备
梯度降温
同种骨
生物力学
Preparation
Gradient freezing
Biomechanics
Bone allograft
