摘要
AIM:To investigate the expression of NF-κBp65 protein and human telomerase reverse transcriptase (hTERT) and their correlation in gastric cancer and precancerous lesions.METHODS: Forty-one patients with primary gastric cancer,15 with dysplasia, 23 intestinal metaplasia and 10 with normal gastric mucosa were included in this study.Expression of NF-κBp65 protein,hTERT mRNA and protein were determined by immunohistochemistry and in situ hybridization.RESULTS:The rate of p65 expression in normal gastric mucosa, intestinal metaplasia,dysplasia and carcinoma was 0%, 34.78%, 53.33% and 60.98%,respectively,while the rate of hTERT mRNA expression was 10.00%,39.13%,66.67% and 85.37% and the rate of hTERT protein expression was 0%,30.43%,60.00% and 78.05%,respectively.All the three parameters were significantly increased in dysplasia and carcinoma compared to normal mucosa, while the expression levels were also significantly higher in carcinoma than in intestinal metaplasia (P<0.05).In gastric cancer tissues, nuclear staining rates of p65 and hTERT protein were both significantly associated with the degree of differentiation, lymph node metastasis,clinical stage and invasion depth (P<0.05).However,hTERT mRNA expression was only significantly associated with clinical stage.There was a positive correlation between p65 and hTERT mRNA(rs=0.661-0.752, P<0.01),and between hTERT protein and hTERT mRNA (rs=0.609-0.750, P<0.01).CONCLUSION: NF-κBp65 and hTERT expressions are upregulated at the early stage of gastric carcinogenesis.NF-κB activation may contribute to hTERT expression and thereby enhance telomerase activity, which represents an important step in carcinogenesis progress.
AIM:To investigate the expression of NF-κBp65 protein and human telomerase reverse transcriptase (hTERT) and their correlation in gastric cancer and precancerous lesions. METHODS:Forty-one patients with primary gastric cancer, 15 with dysplasia,23 intestinal metaplasia and 10 with normal gastric mucosa were included in this study.Expression of NF-κBp65 protein,hTERT mRNA and protein were determined by immunohistochemistry and in situ hybridization. RESULTS:The rate of p65 expression in normal gastric mucosa,intestinal metaplasia,dysplasia and carcinoma was 0%,34.78%,53.33% and 60.98%,respectively,while the rate of hTERT mRNA expression was 10.00%,39.13%, 66.67% and 85.37% and the rate of hTERT protein expression was 0%,30.43%,60.00% and 78.05%,respectively.All the three parameters were significantly increased in dysplasia and carcinoma compared to normal mucosa,while the expression levels were also significantly higher in carcinoma than in intestinal metaplasia (P<0.05).In gastric cancer tissues,nuclear staining rates of p65 and hTERT protein were both significantly associated with the degree of differentiation,lymph node metastasis,clinical stage and invasion depth (P<0.05).However,hTERT mRNA expression was only significantly associated with clinical stage.There was a positive correlation between p65 and hTERT mRNA (r_s=0.661-0.752,P<0.01),and between hTERT protein and hTERT mRNA (r_s=0.609-0.750,P<0.01). CONCLUSION:NF-κBp65 and hTERT expressions are upregulated at the early stage of gastric carcinogenesis. NF-κB activation may contribute to hTERT expression and thereby enhance telomerase activity,which represents an important step in carcinogenesis progress.
