摘要
AIM:To evaluate the effects of p53 on apoptosis and proliferation of hepatocellular carcinoma (HCC) cells treated with transcatheter arterial chemoernbolization (TACE).METHODS: A total of 136 patients with HCC received TACE and other management before surgery were divided into TACE group and non-TACE group.TACE group included 79 patients who had 1-5 courses of TACE before surgery,of them,11 patients had 1-4 courses of chemotherapy (group A),33 patients had 1-5 courses of chemotherapy combined with iodized oil (group B), 23 patients had 1-3 courses of chemotherapy,iodized oil and gelatin sponge (group C),12 patients had 1-3 courses of chemotherapy combined with iodized oil,ethanol and gelatin sponge (group D).Non-TACE group included the remaining 57 patients who had surgery only.The extent of apoptosis was analyzed by transferase mediated dUTP nick end labeling (TUNEL) staining.The expressions of p53, Bcl-2,Bax,Ki-67 and PCNA protein were detected by immunohistochemical method.RESULTS: P53 protein expressions in trabecular and clear cells in HCC specimens were significantly lower than that in pseudoglandar, solid, poorly differentiated or undifferentiated and sclerosis HCC (P<0.05) Expression of p53 protein in HCC cells increased with the increase of pathological grades(P<0.05),and correlated positively with expressions of Ki-67 and PCNA protein,and negatively with Bcl-2 to Bax protein expression rate and AI (P<0.05).Expression of p53 protein was significantly higher in group A than in groups B, C, D and the non-TACE group, and was higher in group B than in groups C and D,and lower in group D than in the non-TACE group (P<0.05).CONCLUSION:Expression of p53 protein can enhance proliferation of HCC cells and suppress apoptosis of HCC cells after TACE.
AIM:To evaluate the effects of p53 on apoptosis and proliferation of hepatocellular carcinoma (HCC) cells treated with transcatheter arterial chemoembolization (TACE). METHODS:A total of 136 patients with HCC received TACE and other management before surgery were divided into TACE group and non-TACE group.TACE group included 79 patients who had 1-5 courses of TACE before surgery,of them,11 patients had 1-4 courses of chemotherapy (group A),33 patients had 1-5 courses of chemotherapy combined with iodized oil (group B),23 patients had 1-3 courses of chemotherapy,iodized oil and gelatin sponge (group C),12 patients had 1-3 courses of chemotherapy combined with iodized oil,ethanol and gelatin sponge (group D).Non-TACE group included the remaining 57 patients who had surgery only.The extent of apoptosis was analyzed by transferase mediated dUTP nick end labeling (TUNEL) staining.The expressions of p53,Bcl-2,Bax,Ki-67 and PCNA protein were detected by immunohistochemical method. RESULTS:P53 protein expressions in trabecular and clear cells in HCC specimens were significantly lower than that in pseudoglandar,solid,poorly differentiated or undifferentiated and sclerosis HCC (P<0.05).Expression of p53 protein in HCC cells increased with the increase of pathological grades (P<0.05),and correlated positively with expressions of Ki- 67 and PCNA protein,and negatively with Bcl-2 to Bax protein expression rate and AI (P<0.05).Expression of p53 protein was significantly higher in group A than in groups B,C,D and the non-TACE group,and was higher in group B than in groups C and D,and lower in group D than in the non-TACE group (P<0.05). CONCLUSION:Expression of p53 protein can enhance proliferation of HCC cells and suppress apoptosis of HCC cells after TACE.
基金
Supported by the National Natural Science Foundation of China,No.30070235
