提高外源基因在巴斯德毕赤酵母中表达量的研究进展

The Study on Improving Expression Levels of Heterologous Gene in Pichia pastoris

巴斯德毕赤酵母 (Pichiapastoris)表达系统是基因工程研究中广泛使用的真核表达系统 ,与现有的其它表达系统相比 ,巴斯德毕赤酵母在表达产物的糖基化修饰、折叠、加工、外分泌及表达量等方面有明显的优势。外源基因在该系统中表达时 ,由于受基因内部的结构、分泌信号、甲醇诱导的浓度及诱导时间、培养温度、启动子、表达环境的 pH值等诸多因素的影响 ,一些外源蛋白的表达也存在着表达不够稳定、表达量较低 ,甚至不表达的情况。对影响巴斯德毕赤酵母表达的各种可能因素进行了分析 ,结合具体实践经验 ,就如何提高外源基因在巴斯德毕赤酵母中表达量的问题进行了综述。

As a eukaryote expression system, Pichia pastoris has been widely used in genetic engineering, which has many merits in the gene expression, protein process and secretion. The gene expression is influenced by a number of factors, such as the structure of gene, secretion signal peptides, methanol concentration, induction phase, temperature, PH, and promoter etc. These factors make some heterologous genes express unstably or express a little. This article analyzes these factors and reviews how to improve expression levels of heterologous genes in Pichia pastoris.