新型不对称三齿多吡啶配体及其混配钌(II)配合物的合成、表征及与DNA相互作用研究

Syntheses, Characterization and DNA-binding Studies of Novel Asymmetric Tridentate Polypyridine Ligands and Their Heteroleptic Ruthenium(II) Complexes

合成了两个新型不对称三齿多吡啶配体 ,3 ( 1,10 菲咯啉基 2 ) 1,2 ,4 三唑 (PHT) ,3 ( 1,10 菲咯啉基 2 ) 5 甲基 1,2 ,4 三唑 (PHMT) ,及其混配配合物 [Ru(tpy) (PHT) ] 2 + (Ru1)和 [Ru(tpy) (PHMT) ] 2 + (Ru2 ) ,通过元素分析、FAB MS ,ES MS ,1HNMR ,IR ,UV vis ,发射光谱和电化学对它们进行了表征 .运用电子吸收光谱、竞争性结合实验和粘度测试等方法研究了配合物与DNA的作用机理 ,结果显示它们均是通过静电作用与DNA结合 ,且Ru2与DNA的作用比Ru1与DNA的作用更强 .

Two novel asymmetric tridentate polypyridine ligands with as-triazole, 3-(1,10-phenanthrolin-2-yl)-1,2,4-triazole (PHT) and 3-(1,10-phenanthrolin-2-yl)-5-methyl-1,2,4-triazole (PHMT) have been designed, synthesized, and characterized by microanalyses, FAB-MS, NMR, FT-IR, and UV-vis. Their heteroleptic complexes [Ru^(tpy)(PHT)](ClO 4) 2·H 2O (Ru1) and [Ru(tpy)(PHMT)](ClO 4) 2·2H 2O (Ru2) were synthesized and investigated with microanalyses, ES-MS, NMR and cyclic voltammetry. DNA binding mechanisms of the complexes were studied by electronic absorption spectra, competitive binding, and viscosity measurements. The results indicated that the modes of these two complexes interacting with DNA were electrostatic interaction, and the DNA-binding of Ru2 was stronger than that of Ru1.