当归对肺纤维化大鼠肺形态学及胶原的影响

Influence on morphology and collagen of angelica sinensis on pulmonary fibrosis in rats

目的 观察当归对博莱霉素所致的肺间质纤维化大鼠的肺形态学及胶原的影响 ,探讨其作用机制。方法 36只大鼠随机分为对照组、模型组及当归组。气管内注入博莱霉素复制大鼠肺间质纤维化模型 ,次日当归组每日腹腔内注射当归注射液 15 0mg/kg,于第 7及第 2 8天每组分别处死 6只大鼠 ,观察肺间质纤维化形成的病理变化及血清Ⅰ型胶原的含量。结果 当归能明显减轻肺泡炎和肺纤维化程度 ,与模型组比较 ,当归组血清Ⅰ型胶原含量于第 7、 2 8天明显降低 (P均 <0 0 5 )。结论 当归能减慢肺间质纤维化的进程 ,对肺间质纤维化有一定的保护作用。

Objective To observe the influence on morphology and collagen of Angelica Sinensis on bleomycin-induced pulmonary fibrosis in rats and to explore its pathogenesis.Methods Thirty-six SD rats in good conditions were divided into three groups at random: control group,model group and Angelica Sinensis group.On experimental day(day 0),the rats were intratracheally instilled with bleomycin,and then treated with Angelica Sinensis 150mg·kg -1 ·d -1 by intraperitoneal injection.On the 7th and 28th day,six rats of each group were killed and the lungs were harvested for histopathological examination and blood was for determinations of collageni.Results Angelica Sinensis could obviously reduced alveolitis and fibrosis.The content of collagenⅠfrom blood in the 7th and 28th day in Angelica Sinensis group was decreased compared with model group satistically (P<0.01 and 0.05 respectively).Conclusion Angelica Sinensis may alleviate the process of pulmonary fibrosis of rats induced by bleomycin and has protective effect on rats with pulmonary fibrosis.