IL-8 IL-17与支气管哮喘发病关系的研究

Role of Interleukin-8 and Interleukin-17 in Pathogenesis of Asthma

目的 探讨白介素 - 8(IL - 8)及白介素 - 17(IL - 17)在支气管哮喘发病机制中的作用。方法 搜集 30例支气管哮喘急性发作期患者治疗前后及其缓解期和 2 0例健康者的静脉血 ,离心后取血清 ,采用酶联免疫吸附实验 (ELISA)法测定血清中IL - 8、IL - 17的浓度 ,同时测定出所有受试者 (急性期治疗前后、缓解期、正常人 )外周血中性粒细胞计数。结果 支气管哮喘急性发作期患者血清IL - 8浓度 (70 1 5 3± 12 1 34)pg/L与缓解期 (2 2 8 5 3± 5 7 5 8)pg/L及对照组 (2 0 2 87± 31 14 )pg/L比较 ,均有极显著差异 (P <0 0 1)。支气管哮喘急性发作期患者血清IL - 17浓度 (76 0 1± 4 35 )pg/L与缓解期 (2 0 85± 3 6 8)pg/L及对照组 (16 30± 3 0 8)pg/L比较 ,均有极显著差异 (P <0 0 1)。支气管哮喘急性发作期患者外周血白细胞计数 (12 34±2 9)× 10 9/L与缓解期 (5 9± 1 39)× 10 9/L及对照组 (3 87± 0 83)× 10 9/L比较 ,均有极显著差异 (P <0 0 1)。支气管哮喘急性发作期患者治疗后 ,血清IL - 8浓度 (4 2 2 6 7± 88 9)pg/L明显低于治疗前 ;血清IL -17浓度 (4 4 0 2± 4 82 )pg/L也明显低于治疗前。血清IL - 8、IL - 17浓度与外周血中性粒细胞计数呈正相关(γ =0 6 34、γ =0 5 94 )。

Objective To evaluate the effect of IL-8 and IL-17 in bronchial asthma mechanism. Methods The serum after centrifuging venous blood from 30 bronchial asthma patients who were in acute attack stage, 30 patients who were at stable period and 20 healthy persons was collected. The concentration of IL-8 and IL-17 was measured respectively by ELISA. At the same time, the number of neutrophilia in peripheral blood of all subjects was counted. Results The concentration of IL-8 and IL-17 of acute attack group were obviously higher than those in stable asthma group and healthy group. The number of neutrophilia from unstable asthmatics was higher than that in stable and normal groups. The level of IL-8 and IL-17 dropped distinctly after therapy. The IL-8 and IL-17in serum were positive related to neutrophilia. Conclusion The concentration of the IL-8 and IL-17 of acute attack asthma patients was higher than that of stable period asthma patients significantly. The IL-8 and IL-17 were capable of inducing infiltration of neutrophilia into the asthmatic airways. They were the most important cytokines which leaded to the acute attack of asthma.