载脂蛋白H基因多态性与高血压肾损害及肾实质性高血压

The relationship of apolipoprotein H gene polymorphism with hypertensive renal injury and renal substantial hypertension

目的 探讨载脂蛋白H(ApoH)基因多态性与高血压肾损害及肾实质性高血压的关系。方法 采用PCR -单链构象多态技术 (PCR -SSCP) ,对黑龙江地区 96例高血压 -肾功能正常患者(EH -NRF)、92例高血压 -肾功能衰竭患者 (EH -CRF)和 10 4例肾实质性高血压 -肾功能正常患者 (RH -NRF)、112例肾实质性高血压 -肾功能衰竭患者 (RH -CRF)及 10 0例健康对照者 (NC)的ApoH基因多态性进行检测。结果 黑龙江地区汉族人ApoH基因 3号外显子存在G341A(Ser88Asn)多态 ,EH -NRF及EH -CRF组G341A(Ser88Asn)多态A等位基因频率高于RH -NRF、RH -CRF及NC组 (P <0 .0 5 ,P <0 .0 1) ;EH -CRF组A等位基因频率高于EH -NRF组 (P <0 .0 5 ) ;RH -NRF及RH -CRF组等位基因频率与NC组差异无显著意义。结论 G341A(Ser88Asn)多态A等位基因可能是黑龙江地区汉族人高血压的遗传危险因素 ,但不能用ApoH基因多态性来解释肾性高血压 ,说明高血压并发尿毒症与尿毒症并发高血压存在不同的遗传基础。

Objective To explore the relationship between the polymorphism of apolipoprotein H gene(ApoH) and hypertensive renal injury and renal substantial hypertension.Methods Ninety-six patients with hypertension and normal renal function(EH-NRF)、92 with hypertension and renal failure(EH-CRF)、104 with renal substantial hypertension and normal renal function(RH-NRF)、112 with renal substantial hypertension and renal failure(RH-CRF)and 100 normal controls(NC) in Heilongjiang province were detected by PCR-single strand conformation polymorphism(PCR-SSCP).Results G341A(Ser88Asn) in the third of ApoH in the Hans in Heilongjiang are polymorphic. The polymorphic A allele of G341A (Ser88Asn)in EH-NRF and EH-CRF occurs more frequently than that in RH-NRF、RH-CRF and NC (P<0.05,P<0.01); The frequency of A allele in EH-CRF is higher than EH-NRF (P<0.05); there is no significant difference in allece frequency between RH-NRF、RH-CRF and NC.Conclusions The polymorphic A allele in G341A(Ser88Asn) may be the genetic risk to hypertension in the Hans in Heilongjiang, but this polymorphism of ApoH is not reasonable to explain renal hypertension, which suggests that there exist different genetic background between hypertension with uremia and uremia with hypertension