解脲脲原体感染者与其正常携带者在量值间的区分研究

Quantitative assessment of Ureaplasma urealyticum in infected patients and normal carriers

目的 探讨解脲脲原体 (UU)感染者与其正常携带者数量的相关性。方法 以UU液体培养阳性为基础 ,选择 <2 4h ,2 4h～ ,4 8h～ ,72h～ ,96～ 1 2 0h培养阳性的标本进行培养鉴定药敏定量一步法和荧光聚合酶链反应 (PCR)定量测定。结果 液体培养≤ 4 8h阳性 2 6人 ,其中荧光PCR定量值≥ 1 0 6拷贝 2 2人 (84 .6 2 % ) ;>4 8h阳性 39人 ,荧光PCR定量值 <1 0 6拷贝 35人 (89.74 % )。培养鉴定药敏定量一步法≤ 4 8h培养阳性组与 >4 8h培养阳性组荧光PCR定量值比较 ,P <0 .0 5 ;>4 8～ 1 2 0h培养阳性组荧光PCR定量值比较 ,P >0 .0 5。结论 培养鉴定药敏定量一步法培养≤ 4 8h阳性或荧光PCR定量值≥ 1 0 6拷贝可作为界定感染者标准 ,培养 >4 8h阳性或荧光PCR测定值 <1 0 6拷贝可作为正常携带。

Objective To evaluate the quantitative difference between Ureaplasma urealyticum infected by patients and carried by normal people. Methods Based on the initial positive culture of Ureaplasma urealyticum, samples were assayed by fluorescence quantitative PCR detection as well as one step method of culture, identification, drug sensitivity and quantitative analysis during the initial positive culture period of <24 h, 24 h～, 48 h～, 72 h～ and 96～120 h. Results The one step culture of samples of 26 patients was positive within 48 h, and 22 of which had fluorescence PCR quantitative value of no less than 10 6 copies ( 84.62% ); the one step culture of samples of 39 patients were positive beyond 48 h, and 35 of which had fluorescence PCR quantitative value of less than 10 6 copies ( 89.74% ). There was significant difference in fluorescence PCR quantitative value between the above two groups. Conclusion Positive culture within 48 h by one step method or fluorescence PCR quantitative value ≥10 6 copies can be defined as infection,and positive culture beyond 48 h or fluorescence PCR quantitative value <10 6 copies are normal carriers. one step method can be act as the criterion for antimicrobal agents treatment.