肿瘤坏死因子对滋养细胞侵袭性的影响

Effects of exogenous tumor necrosis factor alpha on the invasiveness of human trophoblasts

目的探讨外源性肿瘤坏死因子(TNF-α)及其多克隆抗体对原代滋养细胞侵袭力的影响。方法采用逆转录多聚酶链反应(RT-PCR)方法,检测TNF-α、anti TNF-α、TNF-α+anti TNF-α对原代滋养细胞基质金属蛋白酶(MMP-2)及其组织抑制因子(TIMP-2)表达的影响。结果原代滋养细胞表达MMP-2,经过5 ng/ml的TNF-α、TNF-α+antiTNF-α分别刺激培养原代滋养细胞48 h后,细胞MMP-2的表达显著性提高(P<0.001),50 ng/ml的antiTNF-α刺激培养后MMP-2的表达显著性降低(P<0.001);各组间MMP-2的表达存在显著性差异(P<0.001),但是均未见明显的TIMP-2的表达。结论外源性细胞因子TNF-α可以增强滋养细胞的侵袭力,多克隆抗体antiTNF-α则降低其侵袭力。

Objective To observe the effect of exogenous tumor necrosis factor alpha (TNF-α) and its polyclonal antibody (anti-TNF-α) on the invasiveness of human trophoblasts in vitro. Methods The effect of exogenous TNF-αand anti-TNF-αon the expression of matrix metalloproteinase-2 (MMP-2) and tissue inhibitor of metalloproteinases-2 (TIMP-2) in human trophoblastic cells was investigated with reverse transcription (RT)-PCR (RT-PCR). Results The trophoblasts cultured in vitro expressed MMP-2 but not TIMP-2. After incubation of the trophoblasts with 5 ng/ml TNF-αand TNF-αplus anti-TNF-αrespectively for 48 h, significant elevation of MMP-2 expression was induced, while the reverse occurred following incubation of the cells with 50 ng/ml anti-TNF-α. None of the treatments induced TIMP-2 expression. Conclusion Exogenous TNF-αmay promote the invasiveness of the trophoblasts, which can be inhibited by anti-TNF-α.