摘要
目的建立葛根芩连汤及其制剂中黄连总生物碱的含量测定方法。方法氧化铝柱纯化,紫外分光光度法350 nm处测定含量。结果葛根芩连汤水提物中总生物碱含量为32.4 mg/g,加样回收率为98.1 % (RSD =2.9%)。葛根芩连微丸中总生物碱含量为19.6 mg/g。结论该法测定黄连总生物碱方法简单、结果准确,可为葛根芩连微丸质量标准、葛根芩连方及其他含黄连的方剂中生物碱的含量测定提供参考。
Objective To determine the total content of alkaloids from Rhizoma coptidis in the traditional Chinese medicine prescription Gegenqinlian. Methods The extract of Gegenqinlian decoction and powder of the minipills were respectively purified with alumina column chromatography, and the total alkaloid content of Rhizoma coptidis in these preparations was determined using ultraviolet spectrophotometry at 350 nm. Results The total alkaloid content was 32.4 mg/g in the aqueous extract of Gegenqinlian decoction and 19.6 mg/g in Gegenqinlian minipills, with a recovery rate of 98.1% (RSD=2.9%). Conclusion The method we employed yields accurate and stable results with much sensitivity for quality control of Gegenqinlian minipills, and also provides reference for study of Gegenqinlian prescription and other prescriptions that contain Rhizoma coptidis.
出处
《第一军医大学学报》
CSCD
北大核心
2004年第4期437-438,共2页
Journal of First Military Medical University
基金
国家自然科学基金面上重点资助项目(39970886)
广东省自然科学基金(001070)~~
关键词
葛根芩连方
黄连总生物碱
含量
测定
column chromatography
ultraviolet spectrophotometry
Gegenqinlian prescription
alkaloids
Rhizoma coptidis