紫外伤害培养对酵母水解液紫外吸收性能的影响

Effect of UV injuring culture on UV absorption capability of yeast hydrolysate

研究了紫外伤害培养对酵母菌生长繁殖的影响及紫外伤害培养后酵母细胞的酶水解条件和酵母水解液的紫外吸收性能。实验结果表明:酵母菌紫外伤害培养时间为60h～72h。紫外伤害培养后酵母细胞的最佳酶水解条件为:质量分数为10%的酵母细胞悬浮液中添加质量分数为2%的蛋白酶(细胞干重),pH6 5,52℃振荡酶水解26h。紫外伤害培养的酵母细胞经酶水解离心后,上清液稀释10倍测定OD365为0 349,是未经紫外伤害培养的酵母菌酶水解液的3 46倍。波长326nm～400nm(防晒黑区)的紫外吸光率>95%。说明紫外伤害培养后的酵母细胞酶水解液是极具开发价值的UVA生物吸附剂。

The effects of UV injuring culture on the growth of yeast,the enzymatic conditions of yeast cells injured culture by UV and UV absorption capability of yeast hydrolysate were studied.The results show that the time needed for UV injuring culture of yeast is 60 h～70 h.The optimum enzymatic conditions are:neutrase 2 %(dry weight of the cells of the 10 % yeast suspension,pH 6.5,52 ℃ and 26 h for hydrolysis time at 26 ℃ upon centrifugation of these hydrolysate of yeast after UV injuring culture,the clear centrifugate was diluted to 1/10 by water and the OD_(365) value of the diluted sample was determined as 0.349,which was the 3.46 times of the sample that did not treated by UV injuring culture.The UV of 320 nm～400 nm absorbency was more than 95 %.It indicates that the hydrolysate of yeast after UV injuring culture was a useful biological absorbent of UVA.