动物细胞凋亡相关基因p53和c-myc在大麦中的染色体定位

Chromosomal Location of Animal Apoptosis Related Genes p53 and c-myc in Barley

以人的原癌基因c myc和抑癌基因p53的cDNA为探针,经Southern杂交证明,它们在大麦基因组中存在同源序列.利用荧光原位杂交技术(FISH)进一步对大麦进行了染色体定位.在大麦有丝分裂中期染色体上p53有3个位点被检出,分别位于2L(第二染色体长臂)、2S(第二染色体短臂)和6L上,相应百分距离为84.27±0.75、46.24±15.44和19.23±0.53;c myc有2个位点被检出,分别位于5L和3S上,百分距离相应为75.90±6.62和47.63±5.20.利用改进的荧光原位杂交技术,使异源单拷贝或低拷贝基因在大麦姐妹染色单体上,同时出现信号的检出率达到了30%以上.

In animals, proto-oncogene c-myc and tumor suppressor gene p53 are related to many processes of PCD. Southern blotting of human p53 and c-myc cDNA probes indicated that their cDNA sequences were homologous to barley. They were further mapped onto chromosomes of barley by fluorescence in situ hybridization (FISH). It showed that there were three signal sites of the p53 and two sites of the c-myc on mitotic metaphase chromosomes of barley. The signal sites of the p53 were located on 2L(long arm of the chromosome 2), 2S(short arm of the chromosome 2) and 6L, their percentage distances were 84.27±0.75, 46.24±15.44 and 19.23±0.53 correspondingly. Those of the c-myc were positioned on 5L and 3S; their percentage distances were 75.90±6.62 and 47.63±5.20 respectively. For the tested heterogeneus single copy genes, over 30% of the detected cells, which appeared signals simultaneously on sister chromatids of the barley, were obtained by technique improvement of FISH.