萍乡显性核不育水稻不育基因的转育研究

A Study on Transferring Sterile Gene of Pingxiang Dominant Genic Male Sterile Rice into Common Rice Varieties

利用萍乡显性核不育水稻不育株高温条件下表现部分可育的特性,在南昌夏季以萍乡显性核不育水稻杂合不育株作父本,与具有不同细胞质来源的常规水稻品种(粤香占、HR-195、85-02、中鉴100、9311、原早7号、超丰早、R402)杂交,各杂交组合及后代分别在江西南昌和海南三亚种植。结果表明,转育后代出现的不育株育性没有中间类型,败育彻底;遗传背景和环境条件不会对转育后代Ms-p基因表达产生影响,转育的不育株系育性能稳定遗传。转育成功的5个不同细胞质源的显性核不育株系在株高、分蘖力、上三叶长度和角度等农艺性状都与原萍乡显性核不育水稻不育株有明显的差异。

Using the character that the sterile plants of PDGMSR could be partially fertile in high temperature environment, the sterile plants of PDGMSR, as pollen providers, were crossed with eight common rice varieties(Yuexiangzhan, HR-195, 85-02, Zhongjian 100, 9311, Yuanzao 7, Chaofengzao, R402) with different cytoplasmic sources, in summer in Nanchang City. The combinations and their offspring were planted at two locations, Nanchang and Sanya respectively. The results showed that the sterile plants descended from the transferred crosses performed sterile absolutely, having no medium style and the expression of gene Ms-p of their offspring was not influenced by the genetic background and environmental conditions; furthermore, the sterility of transferred sterile plants showed stable heredity. The successful transferred five sterile lines crossed with different cytoplasmic sources showed obvious difference from the original PDGMSR in agronomic traits, such as the plant height, the stooling, and the length and angle of top three leaves.