紫黑吸水链霉菌Streptomyces violaceoniger-hygroscopicus发酵代谢产物对几种常见果蔬病原菌的拮抗活性

Antagonistic Activity of Metabolite on Several Important Pathogens of Vegetable-fruit by Streptomyces violaceoniger-hygroscopicus Fermentation

从南昌地区不同土质中采得约300份土样,用平板分离法筛选得到8株放线菌,经分类鉴定,其形态,培养特性,生理生化特性与紫黑吸水链霉菌形似,命名为紫黑吸水链霉菌Streptomycesviolaceoniger-hygroscopicus。用其发酵液对果蔬病原真菌—青椒疫霉病菌Phytophthoracapsici,番茄灰病菌Botrytiscinerea,黄瓜枯萎病菌Fusariumoxysporumf.sp.cucumerinum,黄瓜灰霉病菌BotrytiscinereaPers,苹果黑星病菌Venturiainaeualis,白菜黑斑病菌Alternariabrassicae等进行拮抗实验研究,结果表明有2株菌株的发酵液对青椒疫霉病菌有明显的抑制作用,1株对黄瓜枯萎病菌有较好的抑制作用,1株对番茄灰霉病菌有一定的抑制效果。

Eight strains of Actinomycetes were isolated from 300 soil samples, which were sampled from different places in Nanchang, Jiangxi Province, by plate-separation method. Through identification, these isolated strains are very similar to streptomyces hygroscopicus in shape, culture feature, physiological and biochemical characteristics. So these strains are named as Streptomyces violaceoniger-hygroscopicus S-1, S-2, S-3, S-4, S-5, S-6, S-7 and S-8. Antagonistic activity of their fermentation metabolite was tested against several important pathogens of vegetable-fruit including Phytophthora capsici, Botrytis cinerea, Fusarium oxy-Sporum f.sp.cucumerinum, Botrytis cinerea pers, Verturiainaeualis, Alternaria brassicae. etc. The results of the experimentation showed that of the 8 strains, two were found to have strong antagonistic activity against paytophthora capsici, one against Fusarium oxysporum f.sp.cucumerinum, and one against botrytis cinerea effectively.