摘要
在250 ml摇瓶中研究了氮源对转兔防御素(NP-1)基因小球藻的异养生长和NP-1表达的影响,结果表明,转NP-1基因小球藻异养培养的最适氮源为硝酸钾和酵母粉,二者的最佳浓度分别为0.9和9 g/L,藻细胞密度达5.11 g/L,是不添加硝酸钾时细胞密度的1.55倍,而NP-1表达量基本不变. 5 L生物反应器分批培养结果表明,转NP-1基因小球藻在含有硝酸钾和酵母粉两种混合氮源的培养基中培养时,硝酸钾被快速消耗而有机氮源充足,藻细胞内的叶绿素和蛋白质含量下降,但NP-1表达量基本不变.
The influence of nitrogen sources on the heterotrophic growth of transgenic Chlorella and rabbit defensin (NP-1) expression in a 250 ml flask was investigated. The results showed that KNO3 and yeast extract were the optimal nitrogen sources for the heterotrophic culture of transgenic Chlorella with NP-1 gene. Dry weight cell density of transgenic Chlorella was improved from 3.29 g/L to 5.11 g/L and NP-1 expression capability kept unchanged as the concentration of KNO3 and yeast extract were 0.9 g/L and 9 g/L respectively. Batch culture of transgenic Chlorella with NP-1 gene in a 5 L bioreactor with the optimized medium was conducted and the process characteristics were also analyzed. It was found that KNO3 was consumed quickly and organic nitrogen was enough in the medium, and chlorophyll and protein contents of transgenic Chlorella decreased while NP-1 expression capability kept unchanged.
出处
《过程工程学报》
EI
CAS
CSCD
北大核心
2004年第1期16-21,共6页
The Chinese Journal of Process Engineering
基金
国家863计划海洋生物技术主题资助项目(编号:2002AA629110)
