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脊椎骨骨骺蛋白的细胞转染定位 被引量：6

Localization of sedlin protein in transfected cell
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摘要 目的　利用重组的脊椎骨骨骺蛋白 (Sedlin)的过表达来了解该蛋白质在细胞中的定位。方法　用间接免疫荧光的方法观察Sedlin在细胞中的定位。结果　荧光显微镜观察表明Sedlin分布于细胞核和细胞质中 ,极可能是以膜泡的形式存在。结论　实验结果为进一步了解Sedlin的功能提供了细胞学基础。 Objective To investigate the localization of recombinant sedlin protein in cultured cell line with its overexpression. Methods To observe the localization of sedlin protein with fluorescent microscopy. Results Recombinant sedlin protein was distributed throughout the transfected cell,especially in vesicles. Conclusion The result provides the basis for further studies of function of sedlin protein.
作者 范礼斌
机构地区 安徽医科大学生物学教研室
出处 《安徽医科大学学报》 CAS 2004年第2期127-129,共3页 Acta Universitatis Medicinalis Anhui
关键词 脊椎骨 骨骺蛋白 细胞转染定位 骨软骨发育不良 spine localization osteochondrodysplasias
分类号 R681.1 [医药卫生—骨科学] R394 [医药卫生—医学遗传学]
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参考文献11

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同被引文献78

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引证文献6

二级引证文献8

安徽医科大学学报
2004年 第2期

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