穹隆-海马伞切断及去势大鼠脑内β淀粉样肽1-40表达的变化被引量：5

EXPRESSIVE VARIETIES OF β-AMYLOID 1-40 IN BRAIN OF RATS WITH FIMBRIA/FORNIX TRANSECTION AND OVARIES CUTTING

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摘要 ①目的　观察穹隆海马伞切断及去势大鼠脑内不同部位 β淀粉样肽 1 4 0 (Aβ1 40 )表达的变化 ,了解与阿尔茨海默病 (AD)相关的发病机制。②方法　将大鼠分为穹隆海马伞切断及去势模型组 (模型组 )和假手术组 ,假手术组除不切断海马伞和去除卵巢外 ,其余步骤同模型组。常规处死大鼠取脑组织行Aβ1 40 免疫细胞化学染色 ,观察海马CA1区、皮质区、杏仁复合体区、基底前脑Meynert核等部位Aβ1 40 阳性细胞表达变化。③结果　假手术组大脑各观察区有少量的Aβ1 40 表达。与假手术组比较 ,模型组大脑海马CA1区、大脑皮质区、杏仁复合体区Aβ1 40 阳性细胞数明显增多 (t=3.96 9～ 6 .6 77,P <0 .0 1)。④结论　穹隆海马伞切断加去势可致大鼠脑内多部位Aβ1 40 表达增多 ,此可能与老年性痴呆病理改变有关。 ObjectiveTo observe the expressive varieties of β-amyloid 1-40(Aβ 1-40) in different sections of the rat brain with fimbria/fornix transection and ovaries and to study the pathogenic mechanisms related to Alzheimer's disease(AD). Me- thodsHealthy female Wistar rats were randomly divided into the model group and control group with five rats in each group. The fimbria/fornix transection and ovaries cutting were simultaneously performed for the animal model. The rats were executed and the brain taken conventionally. The Aβ 1-40 immunocytes were stained by using an immunohistochemical technique. The expressions of the Aβ 1-40 masculine cells in the hippocammpi CA1, cortex, amygdala, Meynert of the basal forebrain were observed. Results A little Aβ 1-40 was expressed in the observed sections of the brain in the control group, while the Aβ 1-40 masculine cells in the model group were significantly increased than those in the control group in the hippocammpi CA1, cortex, and amygdala (t=3.969- 6.677,P<0.01). ConclusionFimbria/fornix transection and ovaries cutting can cause the increase of Aβ 1-40 expression in many sections of the brain, which might be related to the cognitive disturbance due to Alzheimer's disease. [

作者郭宗君杜芳张延霞杨学伟郭云良

机构地区青岛大学医学院脑血管病研究所中国人民解放军第

出处《齐鲁医学杂志》 2004年第1期4-5,8,共3页 Medical Journal of Qilu

基金山东省科技厅科研基金资助项目 ( 0 0 3 13 0 110 ) 青岛市科技局科研基金资助项目 (SG970 2 40 3)

关键词穹隆海马伞去势大鼠脑内 β淀粉样肽1—40 表达阿尔茨海默病 fornix fimbria β-amyliod Alzheimer's disease rats, Wistar

分类号 R749.16 [医药卫生—神经病学与精神病学]

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1Scott A, Lyness SA, Zarow C, et al. Neuron loss in key cholinergic and aminergic nuclei in Alzheimer disease: a meta-analysis[J]. Neurobiology Aging, 2003, 24:1.
2Bieber EJ,Cohen DP.Estrogen and hormone replacement therapy;is there a role in the preservation of cognitive function[J]? Int J Fertil Womens Med, 2001,46(4):206.
3Singh M, Meyer EM,Millard WJ,et al.Ovarian steroid deprivation results in a reversible learning impairment and compromised cholinergic function in female SD rats[J].Brain Res,1994,644:305.
4Ute K, Bigl V, Eschrich K, et al. De-afferentation of the septo-hippocampal pathway in rats as a model of the metabolic events in Alzheimers disease[J]. Int J Devl Neuroscience, 2001,19: 263.
5Paxinos G, Watson C. The rat brain in sterrotaxic coordinated[M]. 2nd. Australia: Academic Press Australia,1988. 23～38.
6He YS,Yao ZB.NGF promotes collateral sprouting fiber in the septo-hippocapal transection[J].Brain Res,1990,586:27.
7Morgan C, Bugueno MP, Garrido J, et al. Laminin affects polymerization, depolymerization and neurotoxicity of Aβ peptide[J]. Peptides, 2002, 23:1229.
8Dumery L, Bourdel F, Soussan Y, et al. β-Amyloid protein aggregation: its implication in the physiopathology of Alzheimers disease[J]. Pathol Biol, 2001, 49:72.
9Casaccia-Bonnefil P, Carter BD, Dobrowsky RT, et al. Death of oligodendrocyted by the interaction of nerve growth factor with its receptor[J]. Nature, 1996,383:716.
10Olivieri G,Novakovic M,Savaskan E, et al. The effects of β-estradiol on SHSY5Y neuroblastoma cells during heavy induced oxidative stress neurotoxicity and β-amyloid secretion[J]. Neuroscience, 2002,113(4):849.