唾液腺提取物抗龋作用的实验研究

Anticaries potential of salivary gland extract

目的 :研究唾液腺提取物 (SGE)的防龋效果。方法 :给SD大鼠接种致龋菌 ,饲喂 2 0 0 0致龋饲料 ,造成大鼠龋齿模型 ,在造模的同时 ,给予低、高剂量SGE ( 4 0 0mg/kg和 15 0 0mg/kg) ,并以氟化物作为阴性对照 ,以喂全价颗粒饲料的正常大鼠为空白对照 ,用Keyes计分法评定患龋情况 ,用NBT法测定血细胞SOD活性。SGE对牙钙的溶出量的测定 ,采用电化学人工龋模型在离体条件下进行。结果 :空白对照不形成龋齿 ,SGE的釉质龋、牙本质浅龋和牙本质深龋级的龋齿计分均非常显著低于模型组 (P <0 .0 0 1或P <0 .0 1) ,SGE能明显抑制牙钙的溶出 ,显著提高血液SOD活性。结论 :SGE有与氟化物相似的抗龋作用 ,可能成为一种新型生物抗龋剂。

Objective: To study the anticaries effects of salivary gland extract (SGE). Methods: Dental caries model was established in 80 SD rats by feeding with cariogenic diet 2000 and by infecting with cariogenic bacteria. 20 model rats in each of the 4 experimental group were fed with SGE at 400 mg/kg, 1 500 mg/kg or 5 mg/L of NaF or deionic water respectively,another 20 were fed with ordinary diet as blank control. The anticaries effects were evaluated by Keyes caries scores, SOD activity in blood cells was determined by NBT test. The effect of SGE on dissoluble Ca 2+ from teeth was determined in electrochemical artificial caries model in vitro. Results: caries was not observed in the blank control. According to Keyes caries score, at E, Ds, Dm and Dx levels, the score in SGE groups was significantly lower than that in the model group (P<0.01),and was similar to that in NaF group(P>0.05). SGE obviously enhanced SOD activity (P<0.05) in blood cells, and remarkably inhibited the dissolution of Ca 2+ (P<0.01) from teeth in electrochemical artificial caries model. Conclusions: SGE is as effective as fluoride in anticaries treatment, and it will become a new kind of bio-anticarious medicine.