DNA聚合酶链反应检测石蜡包埋人脑组织中弓形虫的研究

Detection of Toxoplasma Gondii in Paraffin-embedded Brain Tissue By Polymerase Chain Reaction

孕妇感染弓形虫是胎儿先天性畸形的危险因素。本文运用DNA聚合酶链反应检测30例先天性畸形儿尸检石蜡包埋脑组织中弓形虫。结果表明,30例先天性畸形儿石蜡脑组织切片经体外扩增后出现阳性条带者13例,而对照组无1例阳性(P<0.05)。研究结果进一步支持弓形虫感染与胎儿先天性畸形有密切关系。

Infection of pregnant women with Toxoplasma gondii is a risk factor of fetal congenital malformation.Toxoplasma gondii DNA in paraffin-embedded thin-tissue sections was measured by polymerase chain reaction (PCR).The results revealed that the paraffin-embedded tissue sections Toxoplasma gondii DNA sequences after gene amplification in vitro showed that 13 cases were in positive bands in 30 deformity fetus,no case positive band in 10 controls (P<0.05).The study offers further support to the view that infection of Toxoplasma gondii was associated with fetal congenital malformation.