广西侗族群体的三个STR基因座

Three STR Loci in Dong Ethnic Group in Guangxi Province

[目的 ]了解广西侗族三个短串联重复序列 (STR)位点的遗传多态性分布。 [方法 ]EDTA抗凝血样采自 1 2 0名无血缘关系广西侗族个体。酚 氯仿抽提法提取DNA ,多重PCR技术扩增HUMCSF1PO、HUMTPOX和HUMTH0 1三个基因座 ,产物分析采用高分辨性变性聚丙烯酰胺凝胶垂直电泳、银染显影技术。 [结果 ]HUMCSF1PO位点 ,观察到 7个等位基因 ,1 7种基因型 ;HUMTPOX位点 ,观察到 5个等位基因 ,1 1种基因型 ,HUMTH0 1位点 ,观察到 6个等位基因 ,1 6种基因型。 [结论 ]上述三个STR基因座基因型分布与Hardy Weinberg平衡吻合良好。

This study was conducted to investigate the distribution of three STR loci of Dong ethnic group in Guangxi province. EDTA blood specimens were collected from 120 healthy unrelated Dong individuals in Guangxi province, and DNA was extracted by using phynol chloroform method. Tying of HUMCSF1PO, HUMTPOX, HUMTH01 can be accomplished by amplification of genomic DNA by the polymerase chain reaction (PCR) and locus specific primers, separation and detection amplified alleles were determined by high resolution vertical denaturing polyacrylamide gel electrophoresis(PAGE) and silver staining. 7 alleles and 17 genotypes of HUMCSF1PO locus, 5 alleles and 11 genotypes of HUMTPOX locus, 6 alleles and 16 genotypes of HUMTH01 locus were observed. [Conclusion] The allele distribution of the loci was in good agreement of Hardy Weinberg equilibrium.