不同营养途径对阻塞性黄疸大鼠肠系膜淋巴结细菌移位和白蛋白基因转录的影响

Effects of Nutritional Support via Different Routes on Bacterial Translocation and Liver Albumin Gene Transcription in Obstructive Jaundice Rats

目的提供阻塞性黄疸大鼠不同途径营养支持,观察其对阻塞性黄疸大鼠肠粘膜形态、细菌移位、血清白蛋白和肝脏白蛋白基因转录的影响。方法40只雄性SD大鼠,体重240～280g,随机分为4组:假手术组(SHAM)组、胆总管结扎(CBDL)对照组、CBDL+EN组和CBDL+PN组。接受持续5天的等氮、等热量的营养支持。总热量为630kJ·kg-1·d-1,氮量为1.2g·kg-1·d-1。观察大鼠肠系膜淋巴结细菌移位及肠粘膜形态变化。应用RT-PCR方法观察肝细胞白蛋白基因的转录。结果CBDL组大鼠均发生不同程度的肠系膜淋巴结细菌移位,但各组比较无明显差异。EN组大鼠肠粘膜形态明显优于PN组大鼠。无论EN或PN均不能维持阻塞性黄疸大鼠正常的血清白蛋白水平。PN组大鼠肝脏AlbmRNA表达明显高于对照组和EN组。虽然PN组大鼠肝脏DBPmRNA表达也高于对照组和EN组,但无显著差异。结论在维持阻塞性黄疸大鼠肠粘膜形态方面,EN优于PN,而PN较EN能更好地促进阻塞性黄疸大鼠肝脏白蛋白基因转录。

Objective To evaluate the effects of different nutrition support on intestinal mucosa, bacterial translocation,serum albumin, and liver albumin gene transcription in obstructive jaundice rats. Methods Forty male SD rats, weighing 240 ～ 280 g, were randomly divided into four groups: sham-operated (SHAM)group, common bile duct ligation(CBDL)control group, CBDL+EN group, and CBDL+PN group. Isocaloric and isonitrogenous regimens were given to the rats in the EN and the PN groups for five days. Total calorie was 630 kJ·kg-1·d-1 and nitrogen amount was 1.2 g·kg-1·d-1. Bacterial translocation and intestinal mucosa morphology were observed. Liver albumin gene transcription was examined, using RT-PCR method. Results Bacterial translocation to mesenteric lymph nodes occurred in all of the CBDL groups, but showed no significant difference. Intestinal mucosa morphology was better in the EN group than that in the PN group. Neither EN nor PN could maintain normal serum albumin level in the obstructive jaundice rats. The expression of liver Alb mRNA was obviously higher in the PN group than that in the control and EN groups. The expression of liver DBP mRNA was also higher in the PN group than that in the control and EN groups, but without statistical difference. Conclusions EN may be better than PN in maintaining morphology of intestinal mucosa in obstructive jaundice rats,PN may contribute a better liver albumin gene transcription than EN.