日本对虾肝胰腺的细胞培养

Cell Culture From Hepatopancreas of Penaeus japonicus

实验采用 199培养基 ,并在其基础上添加胎牛血清和葡萄糖 ,渗透压用NaCl,CaCl2 ,MgCl2 ,NaHCO3等调节 .分别在 3种 pH值、3种渗透压及 3种Zn2 + 浓度下原代培养日本对虾肝胰腺细胞 ,并以RNA/DNA值为指标来评定其生长状况 .实验结果表明 ,培养日本对虾肝胰腺细胞的适宜 pH值为 7.6、渗透压为 730 μmol/ g ,培养基中添加Zn2 + 浓度为 80 μg/L时 。

Medium 199 was used in this experiment. It was added to the base medium with fetal bovine serum and glucose. The osmotic pressure was adjusted with NaCl, MgCl_2, CaCl_2, NaHCO_3 etc. The improved medium was used to cultivate the primary cells from hepatopancreas of P. japonicus. On this basis, the judgement was on the hepatopancreatic cells in the conditions of different pH, osmotic pressure or addition of Zn^(2+) through RNA/DNA ratio. The results showed the optimized pH and osmotic pressure to cultivate the cells from hepatopancreas of P. japonicus is pH 7.6, osmotic pressure 730 μmol/g. It can improved the growth of cells by adding Zn^(2+) 80 μg/L compared with other kinds of medium.