不同品系小鼠体外受精方法的研究

Study on Methods of In Vitro Fertilization in Various Mouse Strains

对CD 1、B6DF1和C57BL/6小鼠的卵母细胞采用直接、去卵丘细胞、切割透明带法处理后,分别进行体外受精、体外培养和胚胎移植试验。结果表明:CD 1和B6DF1小鼠体外受精率及受孕率均高于近交系C57BL/6小鼠。3个品系小鼠卵母细胞3种不同处理试验,受孕率差异均不显著;去卵丘细胞卵体外受精率(11%～23%)均显著低于卵丘卵母细胞复合体的受精率(34%～60%)和切割透明带卵的受精率(48%～65%),而C57BL/6小鼠的卵经切割透明带后体外受精率显著高于卵丘卵母细胞复合体的受精率。C57BL/6小鼠理想的体外受精方法为切割透明带法,而CD 1、B6DF1小鼠切割透明带卵受精率与其卵丘卵母细胞复合体的受精率差异均不显著,因此这2个品系可直接用卵丘卵母细胞复合体进行体外受精,其方法简单、有效。

Three mouse strains (CD-1, B6DF1, C57BL/6) oocytes were removed cumulus cells or dissected partial zona-pellucida or surrounded by cumulus cells. The techniques of in vitro fertilization (IVF), culture and embryos transfer were used. Results showed that the higher rates of IVF and pregnancy were CD-1 and B6DF1. There was no significant difference of the pregnancy rate in three disposal ways of oocytes in each of three mouse strains. The rates of IVF of the denuded and zona-intact oocytes (11%-23%) were evidently lower than that of oocytes surrounded by cumulus cells (34%-60%). We found the positive effect of partial zona-pellucida dissection (PZD) on the insemination rate (48%-65%), above all the strain of C57BL/6. So this is the best method of IVF of C57BL/6 while the oocytes surrounded by cumulus cells of CD-1, B6DF1 are directly used to IVF.