N_ω-硝基-L-精氨酸和谷氨酸单氨钠对高血压大鼠血浆血管紧张素II的影响

Changes of angiotensin II in plasma of hypertensive rats after intraperitoneal injection of N_ω-nitro-L-arginine and L-glutamic acidmonoammonium salt

目的:观察长期使用L-精氨酸(L-NNA)和谷氨酸单氨钠(MSG)后高血压大鼠血浆血管紧张素II(angiotensinII,ANGII)的变化。方法:建模后每日腹腔注射L-NNA和MSG,于7、30、70d用放免法测定大鼠血浆ANGII的质量浓度。结果:(1)高血压组各时期的血压均明显高于对照组(P<0 05);注射L-NNA组的血压在7d与高血压组无明显差异,但30、70d显著增高(P<0 05);注射MSG组的血压显著低于高血压组和注射L-NNA组,但仍未恢复至正常水平。(2)对照组大鼠各时期血浆ANGII的质量浓度介于(162 6±49 1)与(181 5±37 8)μg/L之间,高血压组大鼠血浆ANGII的质量浓度均高于对照组,7d时无差异,但30、70d差异显著(P<0 05);注射L-NNA后,ANGII的质量浓度升高明显,其中30、70d具有显著差异(P<0 05),而注射MSG组大鼠血浆ANGII的质量浓度明显降低(P<0 05),但仍然高于对照组和高血压组(P<0 05)。结论:一氧化氮可有效的减少外周组织ANG的分泌,其降压作用可能通过有效的降低血液中ANG的质量浓度来实现。

Aim: To investigate the influence on angiotensin II after intraperitoneal injection of N_ω-nitro-L-arginine(L-NNA) and L-glutamic acidmono-ammonium salt(MSG) in hypertensive rats. Methods: We measured the concentrate of angiotensin II in plasma of hypertensive rats after intraperitoneal injection of L-NNA and MSG. Results: (1) Changes of blood press: The blood pressure of the hypertension group was higher than the control group. After injecting L-NNA, the blood pressure increased significantly at 30 d, 70 d. Injecting MSG the blood pressure changed adversely, but the blood pressure was still abnormal. (2) Changes of angiotensin II in plasma: The content of angiotensin II in (plasma) (of) (control) (rats) (was) (from) ((162.6±49.1)) (to) ((181.5±37.8)) (μg/L.) (In) (hypertensive) (rats,) (it)increased significantly in 30 d and 70 d(P<0.01), except in 7 d. After the rats was injected L-NNA, the content of angiotensin II was scared especially in 30 d and 70 d(P<0.01). Although the concentration of angiotensin II was still higher than that of control group and hypertensive group, MSG could reduce angiotensin II in plasma(P<0.01). Conclusion: Nitric oxide may reduce effectively excretion of angiotensin II in plasma, and the depressor function of nitric oxide may be related to the reduction of angiotensin II in blood.