硝酸甘油对心肌细胞凋亡和Fas基因蛋白及mRNA表达的影响

The effect of nitroglycerin on the apoptosis of myocardial and protein and mRNA expression of Fas gene

目的 探讨硝酸甘油对持续缺血和缺血再灌注损伤时心肌细胞凋亡和 Fas基因蛋白及 m RNA表达的影响。方法 将大白鼠随机分为 5组 ,以活结结扎左冠状动脉的前降支 ,分别造成阻断冠脉血流和再灌注。以缺口末端标记法 (TUNEL)标记凋亡细胞 ;以 S- P免疫组化及逆转录聚合酶链反应 (RT- PCR)法分别检测 Fas基因蛋白与 m RNA的表达变化。结果 凋亡细胞原位标记与半定量分析表明 :硝酸甘油处理组与持续缺血组、缺血再灌注组比较心肌细胞凋亡程度明显降低 ,Fas基因蛋白及 m RNA表达明显减少。结论 硝酸甘油预处理能能明显减轻心肌细胞凋亡程度 ;其机制可能与下调 Fas基因蛋白及 m RNA表达有关。

Objective To study the effect of nitroglycerin on the apoptosis of myocardial and protein and mRNA expression of Fas gene in the myocardial cells during continuous ischemia and ischemia reperfusion injury. Methods Wistar rats were randomly divided into 5 groups. Ligate the anterior descending branches of the left coronary artery with slipknot to block the blood flow and reperfuse the myocardium. The apoptotic cell was labeled by TUNEL, and S-P immunohistochemical staining and RT-PCR analysis examined the changes of the protein and mRNA expression of Fas gene respectively. Results In situ apoptotic cell labeling and semiquantitative analysis revealed: compared with continuous ischemia group and ischemia reperfusion injury group, the degree of the apoptosis of the myocardial cells and the protein and mRNA expression of the Fas gene decreased significantly in nitroglycerin-administrated group. Conclusions The precondition with nitroglycerin can lessen the degree of apoptosis of the myocardial cells significantly may be by up-regulating the protein and mRNA expression of Fas gene.