HPLC法测定利胆颗粒中大黄素、大黄酚的含量

Determination of emodin and chrysophanolin Lidan granules by HPLC

目的 建立利胆颗粒中大黄素、大黄酚的含量测定方法。方法 用HPLC法 ,色谱柱 :LU NAC1 8柱 ;流动相 :甲醇 - 0 .1 %磷酸溶液 ( 85∶1 5 ) ;流速 :1 .2ml·min- 1 ;检测波长 :2 5 4nm。结果 大黄素在 3.90～ 39.0 4mg·L- 1 范围内线性关系良好 ,加样回收率为 98.85 %,RSD =1 .0 9%(n =6) ;大黄酚在7.38～ 73.76mg·L- 1 范围内线性关系良好 ,加样回收率为 98.88%,RSD =1 .5 1 %(n =6)。结论 本法简便、准确 ,测定结果可靠 。

Objective To establish a HPLC method for measuring of emodin and chrysophanol in lidan granules. Methods A LUNA C 18 column was used ,the mobile phase was methanol 0.1%phosphoric acid(85∶15),detected wavelength was 254 nm. Results For emodin, the linear range was 3.90～39.04 mg·L -1 and the average recovery was 98.85%( RSD = 1.09%, n =6);For chrysophanol, the linear range was 7.38～73.76 mg·L -1 and the average recovery was 98.88%( RSD = 1.51%, n =6).Conclusion This method is a simple and accurate and the results are credible. HPLC method could be used as determining content of lidan granules.