4种方法在诊断肺结核病的临床应用分析

Study on clinical application of 4 kinds of methods for detection of pulmonary tuberculosis

目的 探讨痰涂片镜检及培养、聚合酶链反应 凝胶电泳法、聚合酶链反应 - 微孔板杂交技术 (PCR -ELISA)、BBL -MGIT快速培养等方法在诊断肺结核的临床意义。方法 以上述 4种检测方法对肺结核组 1 1 5例、非结核肺疾病 41例、健康对照 36例 ,分别进行检测时 ,观察它们的特异性与敏感性。结果 痰涂片镜检及培养、PCR -凝胶电泳法、PCR -ELISA、BBL -MGIT快速培养的特异性分别为1 0 0 %、86%、96.1 %和 1 0 0 %,敏感性分别为 49.6%、73%、69.6%和 5 6.5 %。结论 通过 4种方法对肺结核患者的检测发现 ,PCR -ELISA技术是一项具有敏感性高 ,特异性强的诊断手段。

Objective To study 4 laboratory detection methods and try to evaluate the diagnostic value in pulmonary tuberculosis. Methods Sputum specimens from 115 patients with active pulmonary tuberculosis,41 patients with respiratory system diseases other than tuberculosis and 36 sputum specimens from healthy control were tested by smear or Lowenstein-Jensen culture, PCR-agarose gel electrophoresis, PCR-Microwell plate hybridization-ELISA and BBL-MGIT rapid culture respectively. The results were compared with each other. Results The examination specificity of smearor Lowenstein-Jensen culture ,PCR-agarose gel electrophoresis ,PCR-Microwell plate hybridization-ELISA, BBL-MGIT rapid culture was 100%,86%,97.1%,100% respectively;sensitivity was 49.6%,73%,69·6%,56.5% correspondingly. Conclusion PCR-Microwell plate hybridization-ELISA can be used to test tubercle bacillus DNA quickly and accurately.This assay,because of its high sensitivity and specificity,is an effective method for assistance diagnosis of tuberculosis.