梅毒螺旋体嵌合抗原的重组克隆与表达

Cloning and Expression of Recombinant Treponema Pallidum Embedded Antigen

目的 连接梅毒螺旋体特异抗原TpN17和TpN15的基因,克隆并表达此嵌合抗原。方法 PCR分别克隆TpN17和TpN15的基因,利用T4 DNA连接酶将二者连接在一起并插入到pRSET B质粒中,在BL21(DE3)菌株中用IPTG诱导表达。结果 连接的TpN17-15基因,测序结果与GenBank数据吻合。结论 SDS-PAGE电泳中显示表达的目的蛋白与预期的蛋白分子量一致。

Objective To link the gene of Trepanema pallidum(TP') antigen TpN17 with that of TpN15 together and to clone and express the recombinant embedded antigen. Methods The gene of TpN17 and TpN15 were cloned respectively by PCR and then linked together by using T4 DNA ligase. The linked DNA was inserted into the pRSET B plasmid and expressed in BL21 (DE3)induced by IPTG. Results The linked gene's sequence TpN17-15 DNA was identical with that gotten by dealed with the dates from the GenBank. Conclusion There are good agreement between the predict size and experiment date gotten by SDS-PAGE of the recombinant embedded antigen.