卡介菌多糖核酸对肺结核病人外周血细胞因子mRNA表达的调节

Effects of Bacillus Calmette Guerin-Polysaccharide nucleic acid on expression of interferon-gamma, tumor necrosis factor-alpha and interleukin-10 mRNA in peripheral blood mononuclear cells from patients with pulmonary tuberculosis

目的 :观察活动性肺结核病人外周血单个核细胞 (PBMC)细胞因子信息核糖核酸 (mRNA)表达特点以及卡介菌多糖核酸的调节作用。方法 :活动性肺结核病人和健康志愿者各 15例 ,分离单个核细胞。分 3组进行培养 ,A组为不加刺激物的对照组 ,B组加入结核菌素纯蛋白衍生物 (PPD) ,C组加入卡介菌多糖核酸 (BCG PSN )。检测PBMCγ 干扰素 (IFN γ) ,肿瘤坏死因子 (TNF α)和白细胞介素10 (IL 10 )mRNA表达。结果 :活动性肺结核病人PBMC在A组IFN γmRNA的表达明显低于健康者 ,P <0 .0 1,TNF α与IL 10mRNA的表达与健康人无差异。在B组IFN γmRNA和TNF αmRNA表达与健康人无差异 ;IL 10mRNA的表达增强 ,与健康人比较 ,P <0 .0 1。在C组 ,IFN γmRNA的表达增强 ,仍低于健康人 ,P <0 .0 5 ;TNF αmRNA的表达有增加 ,与健康人比较 ,P >0 .0 5 ,IL 10mR NA的表达无改变。结论 :活动性肺结核病人保护性细胞因子IFN γ减低 ;BCG PSN刺激可提高免疫细胞IFN γmRNA ,但不增高IL

AIM: To observe the expression of mRNA in peripheral blood mononuclear cells (PBMC) from patients with active pulmonary tuberculosis (PTB) and the effects of Bacillus Calmette Guerin Polysaccharide nucleic acid (BCG PSN) on them. METHODS: PBMC were isolated from 15 PTB patients and 15 healthy donors. The cells were divided into three groups: group A (control group), group B (PPD, culture concentration 10 μg·L -1 ), group C (BCG PSN, culture concentration 0.4mg·L -1 ), and cultured in RPMI 1640 at 37 ℃ under 5 % CO 2 for 48 h. The expression of interferon gamma(IFN γ),tumor necrosis factor alpha(TNF α) and interleukin 10 (IL 10) mRNA in PBMC was detected by RT PCR. RESULTS: The expression of IFN γ mRNA in group A from PTB patients was obviously lower than that in healthy donors ( P <0.01 ). The expression of TNF α and IL 10 mRNA in PBMC from PTB patients was similar to that from healthy donors ( P >0.05). There was no difference of the expression of IFN γ and TNF α mRNA in group B PBMC from both PTB patients and healthy donors ( P >0.05); but the expression of IL 10 mRNA in PBMC from PTB patients was significantly enhanced ( P <0.01). The expression of IFN γ mRNA in group C PBMC was enhanced, but still lower than that in healthy donors ( P <0.05). The expression of TNF α mRNA in PBMC was enhanced, compared with that in healthy donors ( P >0.05). There was no change of the expression of IL 10 mRNA. CONCLUSION: The expression of IFN γ mRNA in PTB patients is reduced. BCG PSN can enhance the expression of the IFN γ mRNA in PBMC, but can not enhance that of IL 10 mRNA.