摘要
用差速离心结合不连续蔗糖密度梯度纯化的鸡传染性支气管炎病毒抗原免疫兔和鸡,获得高效价的多克隆免疫血情。经鸡胚胚体乳剂吸收和过DEAE-52纤维素柱提纯其IgG,建立了检测鸡传染性支气管炎病毒抗原的ELISA双抗体夹心法。应用本ELISA对人工感染鸡和传染性支气管炎病鸡群的气管样本的检测,其病毒抗原阳性检出率分别为10O％(20/20)和78％(39/50)。实验结果表明,ELISA双抗体夹心法对鸡传染性支气管炎的早期诊断具有简单、快速、敏感性高、特异性强和重复性好等优点。
A double-antibody sandwich ELISA for determining IBV antigen was established. Using the ELlSA the trachea samples from chickens infected artificially or naturally by IBV were measured, which positive detectable rates were 100% ( 20/20 ) and 78% ( 39/50 ) respectively. This method can be used for rapid and early diagnosis of avian infectious bronchitis.
出处
《中国兽医科技》
CSCD
1992年第4期5-7,共3页
Chinese Journal of Veterinary Science and Technology
