摘要
把伊氏锥虫云南株动基体DNA微环重组于puc19质粒载体,转化入大肠杆菌JM103菌株,获得KDNA全微环克隆。用α-^(32)P-dATP经缺口翻译标记的重组质粒(PTK)能与伊氏锥虫及其KDNA杂交,而不与核DNA杂交。该质粒探针具有高度的敏感性,能检测出10pg的KDNA和100个锥虫体,是快速鉴定伊氏锥虫的理想工具。
The micro-rings of kinetoplast DNA ( KD-NA ) from T.evansi yunnan strain were recomb-ined into pUC_(19) plasmid and transformed into E.coli JM_(103) to acquire the clone of the intact micro-rigs of KDNA. The recombined plasmid labeled with a^(-32) P-dATP by nick-translation hybridized with T.evansi and its KDNA but didn't hybridize with its nuclear DNA. This plasmid probe had so high sensitivity that 10pg KDNA or 100 T. evansi were able to be detected using it.
出处
《中国兽医科技》
CSCD
1992年第9期5-7,共3页
Chinese Journal of Veterinary Science and Technology
基金
国家自然科学基金
国家青年科学基金资助项目
