窖泥微生物总DNA的提取纯化研究

Research on Extraction & Purification of Tolal DNA of Microbes in Pit Mud

窖泥是产生白酒中香味物质的功能菌的生长繁殖载体,白酒中的呈香呈味物质主要是由窖泥微生态中的己酸菌、丁酸菌、甲烷菌等代谢产生和酯化生化反应生成。从窖泥中提取微生物总DNA,经纯化、PCR扩增等处理分析,可跟踪检测不同时期、不同轮次发酵后窖泥中的各菌种、菌群的变化,对养窖护窖、发酵过程控制、提高发酵糟醅质量和酒质等生产都具有指导意义。

Pit mud was regarded as the carrier for propagation and growth of functional bacteria and functional bacteria could produce flavoring substances in liquor.The aroma-producing substances in liquor were developed mainly through the metaboly and esterifying action and biochemical actions of caproate bacteria,butyrate bacteria and methane bacteria etc.in pit mud.Microbial DNA,extracted-from pit mud and treated by purification and PCR treatment etc.,could be used in tracing detection of bacterial species and bacterial groups changes in pit mud in different periods or in different fermentation stages.The application of this method could guide pits main-tenance,fermentation control,and improvement of fermented grains quality and liquor quality etc.in practice.(Tran.by YUE Yang)