芸苔素内酯含量及部分中间体的高效液相色谱法测定

Analytcal Method of Brassinolide and Part of Middle Products Content by HPLC

试样用1-二甲胺基萘衍生化后,以甲醇+水为流动相,用C18为填充柱和紫外检测器(275nm),对试样中 芸苔素内酯进行高效液相色谱进行分离和测定。

The derivative of brassinolide reacted by 1-Naphthyl methyl ether.mobile phase is acetonitrile/water .Using stainless steel colum that filled the packing of Nucleosil 5 C18. Ultraviolet Detector with 275nm wave length.Determine brassinolide content in formulation by HPLC.